Journal
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
Volume 141, Issue 6, Pages 796-804Publisher
OXFORD UNIV PRESS INC
DOI: 10.1309/AJCP9P2KJSXEKCJB
Keywords
Cervical cancer screening; Self-sampling; HPV genotyping test; Next-generation sequencing (NGS); Ion Torrent PGM; MiSeq
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Funding
- Special Funds for Biotechnology Industry from Shenzhen Development and Reform Commission of China
- Funds for Shenzhen Clinical Molecular Diagnostic Engineering Laboratory from Shenzhen Development and Reform Commission of China
- Preventive Oncology International
- Hologic, Bedford, MA
- Qiagen, Gaithersburg, MD
- Gen-Probe, Bedford, MA
- Merck, Whitehouse Station, NJ
- GE Healthcare, Fairfield, CT
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Objectives: We developed a new human papillomavirus (HPV) genotyping assay based on multiplex polymerase chain reaction and next-generation sequencing (NGS) methods for large-scale cervical cancer screening. Methods: We first trained the assay on 1,170 self-collected samples, balancing the cutoff points for high-risk types. Then using 4,262 separate self-collected specimens, we compared concordance, sensitivity, and specificity for cervical intraepithelial neoplasia type 2 (CIN2) or higher and CIN type 3 (CIN3) or higher of the HPV sequencing assay with that of Hybrid Capture 2 (HC2) direct samples and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry assay self-samples. Results: All assays had a good agreement. The sensitivity for CIN2 or higher and CIN3 or higher of the self-sampling specimens tested with the sequencing assay run on both MiSeq and Ion Torrent Personal Genome Machine sequencer was similar to that of direct-sampling specimens tested with HC2 (P > .05), but the specificity of the sequencing assay for CIN2 or higher and CIN3 or higher was significantly higher than that of HC2 (P < .01). Conclusions: This population-based study has demonstrated the applicability of a new NGS high-risk HPV assay for primary cervical cancer screening based on self-collection.
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