Journal
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
Volume 132, Issue 4, Pages 539-548Publisher
OXFORD UNIV PRESS INC
DOI: 10.1309/AJCPV2I0HGPMGBSQ
Keywords
HER2; Breast cancer; Immunohistochemical analysis; Chromogenic in situ hybridization; CISH; Fluorescence in situ hybridization; FISH
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Funding
- Breast Cancer Now [BREAST CANCER NOW RESEARCH CENTRE] Funding Source: Medline
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Patients with human epidermal growth factor receptor-2 (HER2)+ breast cancer are eligible for trastuzumab treatment: therefore, accurate assessment of HER2 status is essential. Until recently, only 2 methods were validated for determining the HER2 status of breast tumors in the routine diagnostic selling: immunohistochemical analysis and fluorescence in situ hybridization (FISH). Recently bright-field in situ hybridization techniques such as chromogenic in situ hybridization (CISH) and silver-enhanced in situ hybridization (SISH), which combine features of immunohistochemical analysis and FISH, have been introduced for the determination of HER2 status. These new techniques use a peroxidase enzyme-labeled probe with chromogenic defection, instead of fluorescent-labeled probe, allowing results to be visualized by standard bright-field microscopy. Thus, the histologic features and HER2 status of a specimen can be evaluated in parallel. Moreover, signals do not decay over time. This review discusses recent publications regarding CISH and SISH testing, including results scoring and concordance between FISH and immunohistochemical analysis.
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