Journal
JOURNAL OF BIOMEDICAL SCIENCE
Volume 16, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1423-0127-16-13
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Funding
- National Science Council, Taiwan, Republic of China. [NSC 96-2314-B040-005, NSC 97-2314-B040-009]
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Background: Activity of secreted phospholipase A (sPLA2) has been implicated in a wide range of cellular responses. However, little is known about the function of human parvovirus B19-VP1 unique region (VP1u) with sPLA2 activity on macrophage. Methods: To investigate the roles of B19-VP1u in response to macrophage, phospholipase A2 activity, cell migration assay, phagocytosis activity, metalloproteinase assay, RT-PCR and immunoblotting were performed. Results: In the present study, we report that migration, phagocytosis, IL-6, IL-1 beta mRNA, and MMP9 activity are significantly increased in RAW264.7 cells by B19-VP1u protein with sPLA2 activity, but not by B19-VP1uD175A protein that is mutated and lacks sPLA2 activity. Additionally, significant increases of phosphorylated ERK1/2 and JNK proteins were detected in macrophages that were treated with B19-VP1u protein, but not when they were treated with B19-VP1uD175A protein. Conclusion: Taken together, our experimental results suggest that B19-VP1u with sPLA2 activity affects production of IL-6, IL-1 beta mRNA, and MMP9 activity, possibly through the involvement of ERK1/2 and JNK signaling pathways. These findings could provide clues in understanding the role of B19-VP1u and its sPLA2 enzymatic activity in B19 infection and B19-related diseases.
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