Journal
AMERICAN JOURNAL OF BOTANY
Volume 99, Issue 2, Pages 365-371Publisher
WILEY
DOI: 10.3732/ajb.1100309
Keywords
genome sequencing; next-generation sequencing; Poaceae; SNP discovery; transcriptomics; Triticum aestivum; wheat
Categories
Funding
- Australian Research Council [LP0882095, LP0883462, DP0985953]
- Australian Genome Research Facility (AGRF)
- Queensland Cyber Infrastructure Foundation (QCIF)
- Australian Partnership for Advanced Computing (APAC)
- Australian Research Council [LP0882095, DP0985953, LP0883462] Funding Source: Australian Research Council
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Bread wheat (Triticum aestivum; Poaccae) is a crop plant of great importance. It provides nearly 20% of the world's daily food supply measured by calorie intake, similar to that provided by rice. The yield of wheat has doubled over the last 40 years due to a combination of advanced agronomic practice and improved germplasm through selective breeding. More recently, yield growth has been less dramatic, and a significant improvement in wheat production will be required if demand from the growing human population is to be met. Next-generation sequencing (NGS) technologies are revolutionizing biology and can be applied to address critical issues in plant biology. Technologies can produce draft sequences of genomes with a significant reduction to the cost and timeframe of traditional technologies. In addition, NGS technologies can be used to assess gene structure and expression, and importantly, to identify heritable genome variation underlying important agronomic traits. This review provides an overview of the wheat genome and NGS technologies, details some of the problems in applying NGS technology to wheat, and describes how NGS technologies are starting to impact wheat crop improvement.
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