Journal
AMERICAN JOURNAL OF BOTANY
Volume 99, Issue 11, Pages E460-E464Publisher
BOTANICAL SOC AMER INC
DOI: 10.3732/ajb.1200154
Keywords
adaptive genetic differentiation; cross-species amplification; EST-SSR; Liriodendron chinense; Liriodendron tulipifera; Magnoliaceae
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Funding
- Natural Scientific Foundation of China [31270384]
- Chinese Academy of Sciences [KSCX2-EW-Q-16, KSCX2-EW-J-20]
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Premise of the study: A novel set of simple sequence repeat (SSR) markers were developed and characterized from the expressed sequence tag (EST) database of Liriodendron tulipifera for application in population genetic studies of Liriodendron. Methods and Results: Thirty-nine polymorphic EST-SSR loci were identified among 27 individuals sampled from a cultivated population of L. tulipifera. The number of alleles per locus ranged from three to 18. The average observed heterozygosity and expected heterozygosity were 0.684 and 0.778, respectively. Of the 39 loci, 32 showed interspecific transferability and polymorphism in a related species, L. chinense. The number of alleles per locus ranged from two to 11, and the average observed heterozygosity and expected heterozygosity were 0.475 and 0.736, respectively. Conclusions: The developed EST-SSR markers will be useful for investigating adaptive genetic differentiation in Liriodendron.
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