Journal
AMERICAN JOURNAL OF BOTANY
Volume 99, Issue 1, Pages E7-E10Publisher
BOTANICAL SOC AMER INC
DOI: 10.3732/ajb.1100425
Keywords
Arecaceae; date palm; Phoenix dactylifera; sex determination; sex-specific markers
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Funding
- Qatar Foundation
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Premise of the study: Date palm (Phoenix dactylifera) is one of the oldest cultivated trees and is critical to the development of arid land. The date palm is a dioecious monocot with separate male and female trees. This presents a challenge in development as it is impossible to distinguish trees until they flower approximately five to eight years after planting. Methods and Results: We have developed PCR-based assays capable of sex differentiation in multiple date palm cultivars. The primers are designed across gender-specific polymorphisms and demonstrated greater than 90% accuracy in distinguishing date palm gender across multiple varieties. Conclusions: These results indicate that the primers should be helpful in rapidly distinguishing date palm gender from the earliest stages that DNA can safely be collected. This is a vast savings in time over present approaches.
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