Journal
AMERICAN JOURNAL OF BOTANY
Volume 99, Issue 9, Pages E353-E356Publisher
BOTANICAL SOC AMER INC
DOI: 10.3732/ajb.1200053
Keywords
black pod disease; microsatellite markers; Phytophthora megakarya; population genetics; pyrosequencing
Categories
Funding
- CIRAD
- AIP BioRessources EcoMicro
- USDA
Ask authors/readers for more resources
Premise of the study : Phytophthora megakarya is the agent of black pod disease of cacao and is the main pathogen of this crop in Africa. Population genetic studies are required to investigate how this pathogen emerged. To this end, we developed 12 novel polymorphic microsatellite markers for P. megakarya. Methods and Results: Microsatellite sequences were obtained by pyrosequencing of multiplex-enriched libraries. Candidate loci with di- or trinucleotide motifs were selected, and primer pairs were tested with nine P. megakarya isolates. The 12 most polymorphic and unambiguous loci were selected to develop three multiplex PCR pools. The total number of alleles varied from two to nine, depending on loci, and higher than expected heterozygosity was observed. Conclusions: These markers were used for population genetic studies of P. megakarya in Cameroon and for comparison with reference strains from West Africa. This is the first time that microsatellite markers have been developed for P. megakarya.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available