MICROSATELLITES FOR TETRACENTRON SINENSE (TROCHODENDRACEAE), A TERTIARY RELICT ENDEMIC TO EAST ASIA

Premise of the study: Tetracentron sinense (Trochodendraceae) is a Tertiary relict endemic to East Asia. Microsatellite markers were developed and characterized to investigate the population genetics of the species. Methods and Results: Microsatellite markers were isolated from the genome of T. sinense using the protocol of Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO). Eight polymorphic microsatellite markers were assessed in 44 samples collected from three wild populations. The number of alleles observed for each locus ranged from two to five. The observed and expected heterozygosities ranged from 0.0000 to 0.9375 and 0.0000 to 0.7681, respectively. Conclusions: The microsatellite markers will be helpful in further studies of the population genetics and phylogeography of T. sinense