Journal
AMERICAN JOURNAL OF BOTANY
Volume 98, Issue 12, Pages E372-E374Publisher
WILEY
DOI: 10.3732/ajb.1100306
Keywords
chloroplast haplotype; chocolate; Malvaceae; microsatellites; Theobroma cacao; Trinitario
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Funding
- World Bank
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Premise of the study: Chloroplast microsatellites were developed in Theobroma cacao to examine the genetic diversity of cacao cultivars in Trinidad and Tobago. Methods and Results: Nine polymorphic microsatellites were designed from the chloroplast genomes of two T. cacao accessions. These microsatellites were tested in 95 hybrid accessions from Trinidad and Tobago. An average of 2.9 alleles per locus was found. Conclusions: These chloroplast microsatellites, particularly the highly polymorphic pentameric repeat, were useful in assessing genetic variation in T. cacao. In addition, these markers should also prove to be useful for population genetic studies in other species of Malvaceae.
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