Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 78, Issue -, Pages 417-428Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2015.04.021
Keywords
Amorphous aggregates; beta-Sheet; Circular dichroism; Conalbumin; Transmission electron microscopy
Funding
- Council of Scientific and Industrial Research (CSIR) New Delhi, India [37(1456)/10/EMR-II]
- CSIR, New Delhi, India
- University Grants Commission (UGC) New Delhi [39-277/2010]
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Here we have used five non-fluorinated cosolvents (acetonitrile, ethanol, methanol, sec-butanol and ter-butanol) at increasing concentrations and analyzed their aggregation inducing behavior on interaction with conalbumin (CA). The aggregates were identified as amorphous by performing spectroscopic experiments like circular dichroism and dye binding assay. The amorphous aggregate contains rich beta-sheet content, show insignificant increment in Thioflavin-T (ThT) fluorescence intensity but strong 1-anilino-8-napthalene sulfonate (ANS) binding with enhanced fluorescence intensity. We also performed transmission electron microscope (TEM) and scanning electron microscope (SEM) imaging of the aggregates which made the result more informative. The morphology appeared on TEM imaging shows aggregates but there is no exhibition of fibril formation, as was observed in amyloid induced by 2,2,2-trifuoroethanol (TFE) and 1,1,1,3,3,3-hexafluoro-propan-2-ol (HFIP). SEM imaging also gives the similar results indicating the formation of amorphous aggregates. Web based tools (Waltz and AGGRESCAN) predicted aggregation prone regions in CA which are accountable for the aggregation. (C) 2015 Elsevier B.V. All rights reserved.
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