Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 74, Issue -, Pages 498-506Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2015.01.005
Keywords
Spirulina platensis; Polysaccharides extraction; Optimization; Response surface methodology (RSM)
Funding
- Ramin Agricultural and Natural Resources University
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Polysaccharides from Spirulina platensis algae (SP) were extracted by ultrasound-assisted extraction procedure. The optimal conditions for ultrasonic extraction of SP were determined by response surface methodology. The four parameters were, extraction time (X-1), extraction temperature (X-2), ultrasonic power (X-3) and the ratio of water to raw material (X-4), respectively. The experimental data obtained were fitted to a second-order polynomial equation. The optimum conditions were extraction time of 25 min, extraction temperature 85 degrees C, ultrasonic power 90 W and ratio of water to raw material 20 mL/g. Under these optimal conditions, the experimental yield was 13.583 +/- 0.51%, well matched with the predicted models with the coefficients of determination (R-2) of 0.9971. Then, we demonstrated that SP polysaccharides had strong scavenging activities in vitro on DPPH and hydroxyl radicals. Overall, SP may have potential applications in the medical and food industries. (C) 2015 Elsevier B.V. All rights reserved.
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