Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 79, Issue -, Pages 807-812Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2015.05.056
Keywords
Arabidopsis thaliana; Glucuronoxylan; Tandem mass spectrometry
Funding
- Academy of Finland
- Formas through the WoodWisdom-Net Programme (HemiPop project) [1124281]
- Nordic Forest Research [107]
- Glycoscience Graduate School
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Glucuronic acids in Arabidopsis thaliana xylans exist in 4-O-methylated (MeGlcA) and non-methylated (GlcA) forms at a ratio of about 3:2. The matrix-assisted laser desorption/ionization mass spectrometry analysis of the endoxylanase liberated acidic oligosaccharides from the Arabidopsis inflorescence stem showed that two peaks with GlcA (GlcA-Xyl(4)Ac(1) and GlcA-Xyl(5)Ac(2)) had abnormally high intensities, as well as different tandem mass spectra, than their 4-O-methylated counterparts. These peaks were interestingly enriched in the xylan biosynthesis mutant irx7 and irx9-1. Multi-stages fragmentation analysis using negative ion electrospray-ion trap mass spectrometry indicated that this GlcA was further carrying a pentose residue in the glucuronoxylan-derived oligosaccharide from irx9-1. The structure was also identified in Arabidopsis wild type. The results prove evidence of a new pentose substitution on the GlcA residue of Arabidopsis GX, which is likely present in the primary walls. (C) 2015 Elsevier B.V. All rights reserved.
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