Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 74, Issue -, Pages 318-326Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2014.12.037
Keywords
Soft tissue reconstruction; Injectable hydrogel; Alginate; O-carboxymethyl chitosan; PVA; ADSCs
Funding
- Nano Mission, Department of Science and Technology (DST), Government of India under the Thematic Unit of Excellence program
- Council of Scientific and Industrial Research (CSIR), India [9/963(0025)2K12-EMR-I]
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Injectable, biodegradable scaffolds are required for soft tissue reconstruction owing to its minimally invasive approach. Such a scaffold can mimic the native extracellular matrix (ECM), provide uniform distribution of cells and overcome limitations like donor site morbidity, volume loss, etc. So, here we report two classes of biocompatible and biodegradable hydrogel blend systems namely, Alginate/O-carboxymethyl chitosan (O-CMC) and Alginate/poly (vinyl alcohol) (PVA) with the inclusion of fibrin nanoparticles in each. The hydrogels were prepared by ionic cross-linking method. The developed hydrogels were compared in terms of its swelling ratio, degradation profile, compressive strength and elastic moduli. From these preliminary findings, it was concluded that Alginate/O-CMC formed a better blend for tissue engineering applications. The potential of the formed hydrogel as an injectable scaffold was revealed by the survival of adipose derived stem cells (ADSCs) on the scaffold by its adhesion, proliferation and differentiation into adipocytes. Cell differentiation studies of fibrin incorporated hydrogel scaffolds showed better differentiation was confirmed by Oil Red O staining technique. These injectable gels have potential in soft tissue regeneration. (C) 2014 Elsevier B.V. All rights reserved.
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