Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 7, Issue 34, Pages 18920-18923Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b06250
Keywords
antibody; nanomedicine; drug delivery; toxicity; breast cancer
Funding
- Biotechnology and Biological Sciences Research Council [BB/J008656/1]
- Worldwide Cancer Research [12-1054]
- EU FP7-ITN Marie-Curie Network programme RADDEL [290023]
- European Metrology Research Program, Engineering and Physical Sciences Research Council [NEW02-REG3, EP/I014470/1]
- Wellcome Trust Medical Engineering Centre
- Graduate School International Research Award (GSIRA)
- Biotechnology and Biological Sciences Research Council [BB/J008656/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/I014470/1] Funding Source: researchfish
- Worldwide Cancer Research [12-1054] Funding Source: researchfish
- BBSRC [BB/J008656/1] Funding Source: UKRI
- EPSRC [EP/I014470/1] Funding Source: UKRI
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Polyethylene glycol-functionalized nanographene oxide (PEGylated n-GO) was synthesized from alkyne-modified n-GO, using solvent-free click-mechanochemistry, i.e., copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The modified n-GO was subsequently conjugated to a mucin 1 receptor immunoglobulin G antibody (anti-MUC1 IgG) via thiolene coupling reaction. n-GO derivatives were characterized with Fourier-transformed infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), Bradford assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and atomic force microscopy (AFM). Cell targeting was confirmed in vitro in MDA-MB-231 cells, either expressing or lacking MUC1 receptors, using flow cytometry, confocal laser scanning microscopy (CLSM) and multiphoton (MP) fluorescence microscopy. Biocompatibility was assessed using the modified lactate dehydrongenase (mLDH) assay.
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