Journal
AIDS
Volume 23, Issue 8, Pages 897-906Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/QAD.0b013e328329f97d
Keywords
body-dependent cell cytotoxicity; Fc gamma R; HIV controller; humoral immunity; neutralizing antibodies
Categories
Funding
- Agence pour la Recherche Contre le SIDA (ANKS)
- Ensemble contre le SIDA (SIDACTION)
- INSERM
- University Paris-Sud
- NIH [AI 0678501, AI61734]
- Bill and Melinda Gates Foundation [38619, 38643]
- NIH/NIAID CHAVI [U01AI067854]
- NIH/NIAID [AI007392-17]
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Objective: To determine the spectrum of antiviral antibodies in HIV-1-infected individuals in whom viral replication is spontaneously undetectable, termed HIV controllers (HICs). Design: Multicenter French trial ANRS EP36 studying the viral control in HICs. Methods: Neutralizing Antibody (nAb) activities (neutralization assay, competition with broadly reactive monoclonal antibodies, and reactivity against the viral MPER gp41 region), Fc gamma R-mediated antiviral activities, anti body-dependent cell cytotoxicity (ADCC), as well as autoantibody levels, were quantified in plasma from 22 controllers and from viremic individuals. The levels of these different antibody responses and HIV-specific CD8 T cell responses quantified by enzyme-linked immunosorbent spot (ELISPOT) IFN gamma assay were compared in each controller. Results: The levels of antibody against the gp120 CD4 binding site, gp41, as well as Env epitopes near to the sites bound by broadly nAbs 2F5 and 1b12 were not different between HICs and viremic individuals. We did not find significant autoantibody levels in HICs. The magnitude and breadth of nAbs were heterogeneous in HICs but lower than in viremic individuals. The levels of nAbs using Fc gamma R-mediated assay inhibition were similar in both groups. Regardless of the type of antibody tested, there was no correlation with HIV-specific CD8 T cell responses. ADCC was detectable in all controllers tested and was significantly higher than in viremic individuals (P < 0.0002). Conclusion: There was no single anti-HIV-1 antibody specificity that was a clear correlate of immunity in controllers. Rather, for most antibody types, controllers had the same or lower levels of nAbs than viremic individuals, with the possible exception of ADCC antibodies. (C) 2009 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins
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