Journal
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
Volume 45, Issue 5, Pages 529-532Publisher
ELSEVIER
DOI: 10.1016/j.ijantimicag.2014.12.029
Keywords
Pseudomonas aeruginosa; Carbapenem resistance; OprD porin; Cystic fibrosis
Funding
- French 'Ministere de l'enseignement superieur et de la recherche'
Ask authors/readers for more resources
The aim of this work was to investigate the impact of single amino acid substitutions occurring in specific porin OprD on carbapenem resistance of cystic fibrosis (CF) strains of Pseudomonas aeruginosa. A PAO1 Delta oprD mutant was complemented with the oprD genes from five carbapenem-resistant CF strains exhibiting very low amounts of mutated OprD porins in their outer membrane despite wild-type levels of oprD transcripts. Compared with wild-type porin from strain PAO1, single amino acid substitutions S403P (in periplasmic loop 8), Y242H, S278P and L345P (in p-sheets 10,12 and 14, respectively) were found to result in reduced amounts of OprD in the outer membrane, increased carbapenem resistance, and slower growth in minimal medium containing gluconate, an OprD substrate, as the sole source of carbon and energy. This indicates that in CF strains of P. aeruginosa, loss of porin OprD may not only result from mutations downregulating the expression of or disrupting the oprD gene, but also from mutations generating deleterious amino acid substitutions in the porin structure. (C) 2015 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available