Journal
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
Volume 45, Issue 2, Pages 183-187Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijantimicag.2014.09.007
Keywords
Colloids; Antibacterial activity; HET-CAM or irritation delay; Antifouling; Dental caries
Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)
- Centro de Tecnologias Estrategicas do Nordeste (CETENE)
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This study aimed to evaluate the cytotoxicity and bactericidal properties of four silver nanoparticle (AgNP) colloids and their ability to inhibit Streptococcus mutans biofilm formation on dental enamel. The cytotoxicity of AgNPs was evaluated based on signs of vascular change on the chorioallantoic membrane using the hen's egg test (HET-CAM). Bactericidal properties and inhibition of S. mutans biofilm formation were determined using a parallel-flow cell system and a dichromatic fluorescent stain. The percentage of viable cells was calculated from regression data generated from a viability standard. AgNP colloids proved to be non-irritating, as they were unable to promote vasoconstriction, haemorrhage or coagulation. AgNP colloids inhibited S. mutans biofilm formation on dental enamel, and cell viability measured by fluorescence was 0% for samples Sl, S2, S3 and S4 and 36.5% for the positive control (diluted 30% silver diamine fluoride). AgNPs are new products with a low production cost because they have a lower concentration of silver, with low toxicity and an effective bactericidal effect against a cariogenic oral bacterium. Moreover, they do not promote colour change in dental enamel, which is an aesthetic advantage compared with traditional silver products. (C) 2014 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
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