Journal
AGING-US
Volume 2, Issue 6, Pages 353-360Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/aging.100162
Keywords
SIRT1; atherosclerosis; endothelium; inflammation
Categories
Funding
- Swiss National Science Foundation [31-114094/1, 310030_130626/1, 3100-068118]
- University of Zurich
Ask authors/readers for more resources
Excessive production of reactive oxygen species (ROS) contributes to progression of atherosclerosis, at least in part by causing endothelial dysfunction and inflammatory activation. The class III histone deacetylase SIRT1 has been implicated in extension of lifespan. In the vasculature, SIRT1 gain-of-function using SIRT1 overexpression or activation has been shown to improve endothelial function in mice and rats via stimulation of endothelial nitric oxide (NO) synthase (eNOS). However, the effects of SIRT1 loss-of-function on the endothelium in atherosclerosis remain to be characterized. Thus, we have investigated the endothelial effects of decreased endogenous SIRT1 in hypercholesterolemic ApoE(-/-) mice. We observed no difference in endothelial relaxation and eNOS (Ser(1177)) phosphorylation between 20-week old male atherosclerotic ApoE(-/-) SIRT1(+/-) and ApoE(-/-) SIRT1(+/+) mice. However, SIRT1 prevented endothelial superoxide production, inhibited NF-kappa B signaling, and diminished expression of adhesion molecules. Treatment of young hypercholesterolemic ApoE(-/-) SIRT1(+/-) mice with lipopolysaccharide to boost NF-kappa B signaling led to a more pronounced endothelial expression of ICAM-1 and VCAM-1 as compared to ApoE(-/-) SIRT1(+/+) mice. In conclusion, endogenous SIRT1 diminishes endothelial activation in ApoE(-/-) mice, but does not affect endothelium-dependent vasodilatation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available