Journal
AGE
Volume 32, Issue 1, Pages 69-77Publisher
SPRINGER
DOI: 10.1007/s11357-009-9115-2
Keywords
Apolipoprotein E; Liver; Hepatocyte; Ageing; Aging; Low-density lipoprotein receptor; Low density-lipoprotein receptor-related protein; Heparan sulfate proteoglycan
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Funding
- Australian National Health and Medical Research Council
- Ageing and Alzheimer's Research Foundation
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Apolipoprotein E (apoE) is associated with aging and some age-related diseases. The majority of apoE is produced by hepatocytes for the receptor-mediated uptake of lipoproteins. Here, the effects of age on the hepatic expression and distribution of apoE and its receptors were determined using immunofluorescence, Western blots, and quantitative PCR in rat liver tissue and isolated hepatocytes. The expression of apoE mRNA and protein was not influenced significantly by aging. Immunofluorescence studies in isolated hepatocytes showed that apoE was more likely to be co-localized with early endosomes, golgi, and microtubules in isolated old hepatocytes. The mRNA expression of the receptor involved in sequestration of apoE, heparan sulfate proteoglycan was reduced in old age, without any significant effect on the expression of either the low-density lipoprotein receptor or low density-lipoprotein receptor-related protein. Old age is associated with changes in hepatic apoE intracellular trafficking and heparan sulfate proteoglycan expression that might contribute to age-related disease.
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