Journal
INTERNATIONAL JOURNAL FOR PARASITOLOGY
Volume 45, Issue 6, Pages 361-365Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2015.02.006
Keywords
Mitochondrial genome; Eimeriidae; Cyclospora cayetanensis; Apicomplexa; Linear genome; Genomics
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Funding
- Ontario Veterinary College, Canada
- Natural Sciences and Engineering Research Council of Canada (NSERC) [DG 400566]
- Centers for Disease Control (CDC) Initiative for Advanced Molecular Detection and Response to Infectious Disease Outbreak, USA
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The near complete mitochondria] genome for Cyclospora cayetanensis is 6184 bp in length with three protein-coding genes (Cox1, Cox3, CytB) and numerous lsrDNA and ssrDNA fragments. Gene arrangements were conserved with other coccidia in the Eimeriidae, but the C. cayetanensis mitochondrial genome is not circular-mapping. Terminal transferase tailing and nested PCR completed the 5'-terminus of the genome starting with a 21 bp A/T-only region that forms a potential stem-loop. Regions homologous to the C. cayetanensis mitochondrial genome 5'-terminus are found in all eimeriid mitochondrial genomes available and suggest this may be the ancestral start of eimeriid mitochondrial genomes. (C) 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
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