Journal
AFRICAN JOURNAL OF BIOTECHNOLOGY
Volume 8, Issue 22, Pages 6036-6039Publisher
ACADEMIC JOURNALS
Keywords
Carbohydrate binding module; xylan binding protein; cell wall modification; cellulose-hemicellulose network; Nicotiana tabacum
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Funding
- Netherlands Foundation for the Advancement of Tropical Research (WOTRO)
- Laboratory of Plant Breeding, Wageningen University, the Netherlands
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Carbohydrate-binding modules have been shown to alter plant cell wall structural architecture. Hence, they have the potential application of being used to engineer the plant to produce tailor-made natural fibers in the cell wall. The Clostridium thermocellum xylanase, Xyn10B, contains two CBMs that belong to family 22 (CBM22). The C-terminal CBM22-2 of the glycoside hydrolase (GH) 10 had been characterized to interact with xylan, a major hemicellulosic component in the secondary cell wall of plants. In this work, the expression of the CBM22-2 in transgenic tobacco plants was evaluated. Histological examinations of the transgenic stems did not reveal marked cell wall phenotype. In addition, there were no observable changes in the height or the appearance of the transgenic plants expressing the CBM22-2 module. The results indicate that the family 22 carbohydrate binding module is not a potential candidate for use in in planta modification of the cell wall.
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