Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 24, Issue 1, Pages 88-94Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2014.11.016
Keywords
Paeoniflorin; Asthma; IL-5; IL-13; IL-17; MAPK
Categories
Funding
- National Natural Science Foundation of China [81102541, 81173390]
- Initial Scientific Foundation of Huashan Hospital, Fudan University [2013QD08]
- Natural Science Foundation of Zhejiang Province [Y2111111]
- Zhejiang Province Traditional Chinese Medicine Health Science and Technology project [2012ZA105]
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Paeoniflorin (PF), one of the major active ingredients of Chinese peony, has demonstrated anti-inflammatory and immunoregulatory effects. However, it has remained unclear whether PF treatment can inhibit allergic inflammation in asthma. In this study, we evaluated the effects of PF on pulmonary function and airway inflammation in asthmatic mice. The allergic asthma models were established in BALB/c mice. The mice were sensitized and challenged with ovalbumin. Airway hyperresponsiveness was detected by direct airway resistance analysis. Lung tissues were examined for inflammatory cell infiltration. IL-5, IL-13, IL-17, and eotaxin in bronchoalveolar lavage fluid (BALF) and their mRNA expression in lung tissue were examined by ELISA and realtime PCR, respectively. The total IgE level in serum was measured by ELISA. The protein expression of p-ERK and p-JNK was detected by western blot. Our data showed that PF oral administration significantly reduced airway hyperresponsiveness to aerosolized methacholine and decreased IL-5, IL-13, IL-17 and eotaxin levels in the BALF, and decreased IgE level in the serum. Histological studies showed that PF administration markedly decreased inflammatory infiltration. Similarly, treatment with PF significantly inhibited IL-5, IL-13, IL-17 and eotaxin mRNA expression in lung tissues. The protein expression levels of p-ERK and p-JNK were substantially decreased after oral administration of PF. In summary, PF displayed anti-inflammatory effects in the OVA-induced asthmatic model by decreasing the expression of IL-5, IL-13, IL-17 and eotaxin. These effects were mediated at least partially by inhibiting the activation of MAPK pathway. (C) 2014 Elsevier B.V. All rights reserved.
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