Journal
THEORETICAL AND APPLIED GENETICS
Volume 120, Issue 1, Pages 85-91Publisher
SPRINGER
DOI: 10.1007/s00122-009-1161-8
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Funding
- National Science Foundation [DBI-0332411]
- USDA Cooperative State Research, Education, and Extension Service-National Research Initiative [03-35300-13104]
- USDA Initiative for Future Agriculture and Food Systems [IFAFS 2000-04292]
- NSF Plant Genome Research Program [DBI-0421630]
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Due to their highly polymorphic and codominant nature, simple-sequence repeat (SSR) markers are a common choice for assaying genetic diversity and genetic mapping. In this paper, we describe the generation of an expressed-sequence tag (EST) collection for the oilseed crop safflower and the subsequent development of EST-SSR markers for the genetic analysis of safflower and related species. We assembled 40,874 reads into 19,395 unigenes, of which 4,416 (22.8%) contained at least one SSR. Primer pairs were developed and tested for 384 of these loci, resulting in a collection of 104 polymorphic markers that amplify reliably across 27 accessions (3 species) of the genus Carthamus. These markers exhibited a high level of polymorphism, with an average of 6.0 +/- A 0.4 alleles per locus and an average gene diversity of 0.54 +/- A 0.03 across Carthamus species. In terms of cross-taxon transferability, 50% of these primer pairs produced an amplicon in at least one other species in the Asteraceae, and 28% produced an amplicon in at least one species outside the safflower subfamily (i.e., lettuce, sunflower, and/or Gerbera). These markers represent a valuable resource for the genetic analysis of safflower and related species, and also have the potential to facilitate comparative map-based analyses across a broader array of taxa within the Asteraceae.
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