Journal
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
Volume 97, Issue -, Pages 90-96Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ibiod.2014.10.017
Keywords
Bacillus; Bioremediation; nfrA; Chromium; Rare-earth ore
Funding
- National Natural Science Foundation [31200085, 31100888]
- Gansu provincial science and technology support program [1104NKCA092]
Ask authors/readers for more resources
Baiyin city, which is located upstream of the Yellow River and in the central part of Gansu province, China, is severely contaminated by heavy metals such as chromium. A Gram-positive bacterium BYCr-1 capable of reducing hexavalent chromium (Cr(VI)) to trivalent (Cr(III)) aerobically was isolated from a rare-earth ore in Baiyin, Gansu, China. 16S rRNA analysis revealed that it was closely related to Bacillus subtilis. It can reduce 0.2 mM Cr(VI) to Cr(III) in M9 medium after 48 h incubation. Transmission electron microscopy (TEM) image showed that Cr(III) precipitates were located both inside and outside the cells. An nfrA gene was upregulated by 53 folds upon Cr(VI) treatment Furthermore, E. coli-NfrA demonstrated elevated chromate-reducing ability. Our results indicate that BYCr-1 is able to resist and reduce high concentrations of Cr(VI), which makes it a potentially suitable candidate for bioremediation of Cr(VI) contamination. This study also reveals that nfrA confers Cr(VI)-reducing ability in Bacillus subtilis. (C) 2014 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available