Journal
ADVANCED SYNTHESIS & CATALYSIS
Volume 353, Issue 13, Pages 2191-2215Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adsc.201100446
Keywords
biocatalysis; directed evolution; enantioselectivity; enzyme catalysis; protein engineering; rational protein design
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Funding
- Alexander von Humboldt Foundation
- Deutsche Bundesstiftung Umwelt
- State Mecklenburg-Vorpommern
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Modern tools for enzyme discovery and protein engineering substantially broadened the number of enzymes applicable for biocatalysis and helped to alter their properties such as substrate range, enantioselectivity, and stability under process conditions. In addition, these methods also enabled one to explore reactions for organic synthesis for which no suitable enzymes were available until recently. This review provides a summary of the different concepts and technologies, which are exemplified for various enzymes.
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