Journal
ADVANCED SYNTHESIS & CATALYSIS
Volume 351, Issue 9, Pages 1303-1311Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adsc.200800727
Keywords
bile acids; hydroxysteroid dehydrogenases; one-pot reaction; oxido-reductions; regioselectivity
Categories
Ask authors/readers for more resources
The hydroxysteroid dehydrogenases (HSDHs)-catalyzed one-pot enzymatic synthesis of 12-ketoursodeoxycholic acid (3 alpha,7 beta-dihydroxy-12oxo-5 beta-cholanoic acid), a key intermediate for the synthesis of ursodeoxycholic acid, from cholic acid has been investigated. This goal has been achieved by alternating oxidative and reductive steps in a one-pot system employing HSDHs with different cofactor specificity, namely NADH-dependent HSDHs in the oxidative step and an NADPH-dependent 7 beta-HSDH in the reductive one. Coupled in situ regeneration systems have been exploited not only to allow the use of catalytic amounts of the cofactors, but also to provide the necessary driving force to opposite reactions (i.e., oxidation and reduction) acting on different sites of the substrate molecule. Biocatalysts suitable for the set-up of this process have been selected and their kinetic behaviour in respect of. the reactions of interest has been evaluated. Finally, the process has been studied employing the enzymes both in free and compartmentalized form.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available