Journal
ADVANCED SYNTHESIS & CATALYSIS
Volume 350, Issue 3, Pages 411-418Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adsc.200700458
Keywords
asymmetric bioreduction; enoate reductase; nitroalkenes; old yellow enzyme; stereocomplementary process; alpha,beta-unsaturated carbonyl compounds
Categories
Ask authors/readers for more resources
Three cloned enoate, reductases from the old yellow enzyme family of flavoproteins were investigated in the asymmetric bioreduction of activated alkenes. 12-Oxophytodienoate reductase isoenzymes OPR1 and OPR3 from Lycopersicon esculentum (tomato), and YqjM from Bacillus subtilis displayed a remarkably broad substrate spectrum by reducing a,p-unsaturated aldehydes, ketones, maleimides and nitroalkenes. The reaction proceeded with absolute chemoselectivity - only the conjugated C=C bond was reduced, while isolated olefins and carbonyl groups remained intact - with excellent stereoselectivities (ees up to > 99%). Upon reduction of a nitroalkene, the stereochemical outcome could be determined via choice of the appropriate enzyme (OPR1 versus OPR3 or YqjM), which furnished the corresponding enantiomeric nitroalkanes in excellent ee. Molecular modelling suggests that this enzyme-based stereocontrol is caused by subtle differences within the active site geometries.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available