Journal
ADVANCED MATERIALS
Volume 24, Issue 41, Pages 5543-5547Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adma.201201805
Keywords
cell patterning; biological complexity; tissue engineering; microstructures; thermoresponsive platforms; multicellular organization; cancer biology; developmental biology; drug discovery
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Funding
- U.S. Army Research Office through the Institute for Soldier Nanotechnologies at MIT [DAAD-19-02-D-002]
- MIT-Portugal Program [MPP-09Call-Langer-47]
- NIH [DE013023, DE016516, HL092836, DE019024, EB012597, AR057837, DE021468, HL099073]
- Office of Naval Research
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A simple method to control spatial organizations of multiple cell types in predefined geometries by using poly(N-isopropylacrylamide)-based dynamic microwells is described. Dynamic microwells are used to pattern two different cell types in a spatially controlled manner by exploiting their shape changing properties at two different temperatures. Both spatial organization of two different cell types and control of pattern geometry are achieved with circular and square dynamic microwells.
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