Facile Conjugation of Biomolecules onto Surfaces via Mussel Adhesive Protein Inspired Coatings

A new surface bioconjugation strategy is presented. A polydopamine surface coating provides chemical activation on material surfaces, is resistant to hydrolysis, and offers selectivity in coupling of biomolecules via nucleophilic groups through simple pH control. Control of orientation of immobilized biomolecules may be possible using terminally modified DNA or His-containing proteins.