Precision Polymers: Monodisperse, Monomer-Sequence-Defined Segments to Target Future Demands of Polymers in Medicine

The established technology platforms of solid-phase-supported oligopeptide and oligonucleotide synthesis can be explained to access fully synthetic macromolecules, preserving both the monodisperse character and the defined monomers sequence. Precision polymers are sequentially assembled from a library of functional building blocks, enabling one to program interaction capabilities or generate functions by sequence-specific positioning of functionalities. Examples are provided, showing that these monodisperse macromolecules can be conjugated to oligonucleotides, oligopeptides, or poly(ethylene glycol)s. The resulting model systems can contribute to the understanding of complex biomedical related process. Due to the absence of chemical and molecular weight distributions in these multifunctional segments, exact correlation of the monomers sequence and (bio)properties is attainable. This is demonstrated by the design of carrier systems that exhibit fine-tuned interactions with plasmid DNA, actively controlling important steps in DNA delivery and transfection, such as polyplex formation, DNA compression, and the release of the cargo.