Rapid Microfluidic Generation of Patterned Aldehydes from Hydroxy-Terminated Self Assembled Monolayers for Ligand and Cell Immobilization on Optically Transparent Indium Tin Oxide Surfaces

Selective immobilization of a wide range of ligands on an indium tin oxide (ITO) surface is demonstrated. A chemoselective immobilization strategy to tailor ITO surfaces is developed by selectively oxidizing hydroxyl-terminated phosphonate self-assembled monolayers (SAMs) to aldehyde-presenting SAMs using micro-fluidic channels and then reacting with oxyamine-containing ligands - all on a chip.