Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 23, Issue 28, Pages 3488-3493Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201202777
Keywords
siRNA; gene silencing; gold nanoparticles; in vivo; poly-L-lysine
Categories
Funding
- NIH [CA135312]
- DOD [W81XWH-11-1-0442]
- Golfers Against Cancer Foundation
Ask authors/readers for more resources
Persistent gene silencing is crucially required for the successful therapeutics of short interfering RNA (siRNA). Here, a nanoparticle-based delivery system is presented which assembles by layering siRNAs between protease degradable polypeptides to extend the therapeutic window. These tightly packed nanoparticles are efficiently taken up by cells by endocytosis, and the fabricated siRNAs are gradually released following intracellular degradation of the polypeptide layers. During cell division, the particles are distributed to the daughter cells. Due to the slow degradation through the multiple layers, the particles continuously release siRNA in all cells. Using this controlled release construct, the in vivo gene silencing effect of siRNA is consistent for an ultralong period of time (>3 weeks) with only a single treatment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available