Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 22, Issue 16, Pages 3389-3398Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201200138
Keywords
cytoprotective coating; hydrogen-bonding; layer-by-layer; antioxidant; polyphenol; immunomodulatory
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Funding
- National Institute of Biomedical Imaging and Bioengineering at the National Institutes of Health [P30EB011319]
- American Diabetes Association [1-09-JF-54]
- Islet Resource Facility of UAB Comprehensive Diabetes Center (UAB IRF-CDC) (NIH) [P60DK79636]
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Though transplantation of pancreatic islet cells has emerged as a promising treatment for Type 1 diabetes its clinical application remains limited due to a number of limitations including both pathogenic innate and adaptive immune responses. This paper reports on a novel type of multifunctional cytoprotective material applied to coat living pancreatic islets. The coating utilizes hydrogen-bonded interactions of a natural polyphenol (tannic acid) with poly(N-vinylpyrrolidone) deposited on the islet surface via non-ionic layer-by-layer assembly. It is demonstrated that the coating is conformal over the surface of mammalian islets including those derived from rat, non-human primate (NHP), and human. In contrast to unmodified controls, the coated islets maintain their viability and beta-cell functionality for at least 96 hours in vitro. It is also determined that the coating demonstrates immunomodulatory cytoprotective properties suppressing pro-inflammatory cytokine synthesis in stimulated bone marrow-derived macrophages and diabetogenic BDC-2.5 T cells. The coating material combines high chemical stability under physiologically relevant conditions with capability of suppressing cytokine synthesis, crucial parameters for prolonged islet integrity, viability, and function in vivo. This study offers new opportunities in the area of advanced multifunctional materials to be used for a cell-based transplantation therapy
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