Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 22, Issue 9, Pages 1940-1951Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201102398
Keywords
bio-nanotechnology; catalysis; magnetic nanoparticles; self-assembly; structure-property relationships
Categories
Funding
- Thai government
- King Abdullah University of Science and Technology (KAUST) [KUS-C1-018-02]
- US Department of Transportation [DTOS59-07-G-00052]
- National Science Foundation [DMR-0520404]
Ask authors/readers for more resources
Bio-nanocatalysts (BNCs) consisting of horseradish peroxidase (HRP) self-assembled with magnetic nanoparticles (MNPs) enhance enzymatic activity due to the faster turnover and lower inhibition of the enzyme. The size and magnetization of the MNPs affect the formation of the BNCs, and ultimately control the activity of the bound enzymes. Smaller MNPs form small clusters with a low affinity for the HRP. While the turnover for the bound fraction is drastically increased, there is no difference in the H2O2 inhibitory concentration. Larger MNPs with a higher magnetization aggregate in larger clusters and have a higher affinity for the enzyme and a lower substrate inhibition. All of the BNCs are more active than the free enzyme or the MNPs (BNCs > HRP MNPs). Since the BNCs show surprising resilience in various reaction conditions, they may pave the way towards new hybrid biocatalysts with increased activities and unique catalytic properties for magnetosensitive enzymatic reactions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available