Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 20, Issue 6, Pages 976-982Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.200901812
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Funding
- 333 Talent Project Foundation
- Qing Lan Project of Jiangsu Province
- Jiangsu Science and Technology Department [2009148, 2008318]
- National Science Foundation of China [20703010]
- China Scholarship Council
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A novel hydrogel suspension array, which possesses the joint advantages of quantum-dot-encoded technology, bioresponsive hydrogels, and photonic crystal sensors with full multiplexing label-free DNA detection capability is developed. The microcarriers of the suspension array are quantum-dot-tagged DNA-responsive hydrogel photonic beads. In the case of label-free DNA detection, specific hybridization of target DNA and the crosslinked single-stranded DNA in the hydrogel grid will cause hydrogel shrinking, which can be detected as a corresponding blue shift in the Bragg diffraction peak position of the beads that can be used for quantitatively estimating the amount of target DNA. The results of the label-free DNA detection show that the suspension array has high selectivity and sensitivity with a detection limit of 10(-9) M. This method has the potential to provide low cost, miniaturization, and simple and real-time monitoring of hybridization reaction platforms for detecting genetic variations and sequencing genes.
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