Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 20, Issue 17, Pages 2770-2777Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201000495
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Funding
- National University of Singapore [ARF R-279-000-234-123, R-279-000-301-646]
- Ministry of Education [R-279-000-255-112]
- Ministry of Defense [R-279-000-301-232]
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A red-fluorescent conjugated polyelectrolyte (CPE, P2) is grafted with dense poly(ethylene glycol) (PEG) chains via click chemistry and subsequently modified with folic acid to form a molecular brush based cellular probe (P4). P4 self-assembles into a core-shell nanostructure in aqueous medium with an average size of 130 nm measured by laser light scattering. As compared to P2, P4 possesses not only a substantially higher quantum yield (11%), but also reduced nonspecific interactions with biomolecules in aqueous medium due to the shielding effect of PEG. In conjunction with its high photostability and low cytotoxicity, utilization of P4 as a far-red/near-infrared cellular probe allows for effective visualization and discrimination of MCF-7 cancer cells from NIH-3T3 normal cells in a high contrast, selective, and nonviral manner. This study thus demonstrates a flexible molecular brush approach to overcome the intrinsic drawbacks of CPEs for advanced bioimaging applications.
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