Journal
INSTRUMENTATION SCIENCE & TECHNOLOGY
Volume 43, Issue 5, Pages 536-544Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10739149.2015.1030678
Keywords
Escherichia coli; laser-induced fluorescence; marine sewage; microfluidic chip
Funding
- Natural Science Foundation of China [51205034]
- Scientific Research Program of the Ministry of Transport [2013329225260]
- Fundamental Research Funds for the Central Universities [3132014332]
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Pathogenic bacteria in sewage from ships are a threat to the marine environment and port security. However, most control regimes do not have an effective method for inspecting sewage; therefore, a suitable instrument is needed. This article reports single cell detection employing laser-induced fluorescence of Escherichia coli in marine sewage from a microfluidic chip with embedded optical fibers. Single E. Coli cells were determined following staining with fluorescein with a signal-to-noise ratio exceeding five.
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