Expression profiles of glutathione S-transferase superfamily in Spodoptera litura tolerated to sublethal doses of chlorpyrifos

Chlorpyrifos (CPF) is a broad-spectrum organophosphate insecticide. Glutathione S-transferases (GSTs) in insects are a family of detoxification enzymes and they play critical roles in CPF detoxification. Spodoptera litura is one of the most destructive agricultural pests in tropical and subtropical areas in the world. In this study, 37 Slgsts from 46 unique transcripts of gsts in S. litura transcriptome data, including eight previously reported GSTs, were identified and their expression patterns in susceptible and 12-generation-CPF-treated strains were analyzed to understand the roles of these Slgsts in sublethal doses of CPF tolerance. The results indicate that the members of the S. litura GST superfamily could be distinguished into three major groups: one group, including six cytosolic Slgsts (SlGSTe1, SlGSTe3, SlGSTe10, SlGSTe15, SlGSTo2 and SlGSTs5) and two microsomal Slgsts (SlMGST1-2 and SlMGST1-3), was directly responsible for CPF induction in both 12-generation-treated and susceptible strains; the second group, including three cytosolic Slgsts (SlGSTe13, SlGSTt1 and SlGSTz1) and one microsomal Slgst (SlMGST1-1), was induced only in the 12-generation-treated strain; the third group, including eight cytosolic Slgsts (two epsilon, three delta, one omega, one zeta and one unclassified Slgst), was expressed 1.52-5.15-fold higher in the 12-generation-treated strain than in the susceptible strain.