The effect of commercial herbicide exposure on the life history and insecticide resistance phenotypes of the major malaria vector Anopheles arabiensis (Diptera: culicidae)

Herbicides, such as atrazine and glyphosate, are common agrochemicals known to pollute surface ground water. As such, aquatic invertebrates associated with agricultural activities can be exposed to varying doses of these xenobiotics. Anopheles arabiensis, a major malaria vector species in southern Africa, is often closely associated with agricultural activities. This study aimed to examine the effects of larval atrazine or glyphosate exposure on larval and adult life history traits on two laboratory strains of An. arabiensis; one insecticide susceptible (SENN), the other selected for resistance (SENN DDT). Atrazine delayed time to pupation in both strains, but markedly more so in SENN DDT. Glyphosate treatment reduced time to pupation in SENN DDT. Larval atrazine exposure decreased adult longevity in SENN, while both herbicide treatments significantly increased adult longevity in SENN DDT. Larval glyphosate exposure was the more potent enhancer of insecticide tolerance in adult mosquitoes. In SENN DDT, it reduced deltamethrin and malathion-induced mortality, and the LT50 s for these insecticides were increased in association with herbicide exposure. Glyphosate exposure also increased the LT50 s for malathion and deltamethrin in SENN. Exposure to both herbicides had contrasting effects on detoxification enzyme activities. Although both increased cytochrome P450 activity, they had opposite effects on those enzymes involved in reactive oxygen species detoxification. Glyphosate decreased glutathione S-transferase activity, but increased catalase activity with atrazine having the opposite effect. This study demonstrates that larval exposure to the herbicides atrazine and glyphosate can affect the insecticide susceptibilities and life history traits of epidemiological importance in An. arabiensis, with glyphosate being the more potent effector of insecticide resistance.