Journal
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
Volume 62, Issue -, Pages 64-74Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2015.03.006
Keywords
Insect immunity; RNA-Seq; Transcriptome; Gene annotation; Expression profiling
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Funding
- NIH [GM58634]
- DARPA/NSF [IOS-1354421]
- NSF [OCI-1126330]
- [OKLO2450]
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1354421] Funding Source: National Science Foundation
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Signal transduction pathways and their coordination are critically important for proper functioning of animal immune systems. Our knowledge of the constituents of the intracellular signaling network in insects mainly comes from genetic analyses in Drosophila melanogaster. To facilitate future studies of similar systems in the tobacco hornworm and other lepidopteran insects, we have identified and examined the homologous genes in the genome of Manduca sexta. Based on 1:1 orthologous relationships in most cases, we hypothesize that the Toll, Imd, MAPK-JNK-p38 and JAK-STAT pathways are intact and operative in this species, as are most of the regulatory mechanisms. Similarly, cellular processes such as autophagy, apoptosis and RNA interference probably function in similar ways, because their mediators and modulators are mostly conserved in this lepidopteran species. We have annotated a total of 186 genes encoding 199 proteins, studied their domain structures and evolution, and examined their mRNA levels in tissues at different life stages. Such information provides a genomic perspective of the intricate signaling system in a non-drosophiline insect. (C) 2015 Elsevier Ltd. All rights reserved.
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