Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 7, Issue 28, Pages 15658-15666Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b04741
Keywords
electrospun cellulose nanofibrous membranes; maleic anhydride; surfaces functionalization; lysozyme; adsorption
Funding
- National Natural Science Foundation of China [51322304]
- Shanghai Committee of Science and Technology [12JC1400101]
- Fundamental Research Funds for the Central Universities
- DHU Distinguished Young Professor Program
Ask authors/readers for more resources
Construction of adsorptive materials for simple, efficient, and high-throughput adsorption of proteins is critical to meet the great demands of highly purified proteins in biotechnological and biopharmaceutical industry; however, it has proven extremely challenging. Here, we report a cost-effective strategy to create carbonyl groups surface-functionalized nanofibrous membranes under mild conditions for positively charged protein adsorption. Our approach allows maleic anhydride to in situ graft on cellulose nanofibrous membranes (CMA) to construct adsorptive membranes with large surface area and tortuous porous structure. Thereby, the resultant CMA membranes exhibited high adsorption capacity of 160 mg g(-1), fast equilibrium within 12 h, and good reversibility to lysozyme. Moreover, the dynamic adsorption was performed under low pressure-drops (750 Pa), with a relatively high saturation adsorption amount of 118 mg g(-1), which matched well with the requirements for proteins purification. Considering the excellent adsorption performance of the as-prepared adsorptive membranes, this simple and intriguing approach may pave a way for the design and development of robust and cost-effective adsorption membranes to meet the great demands for fast and efficient adsorption of positively charged proteins.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available