Journal
ACTA PHYSIOLOGIAE PLANTARUM
Volume 36, Issue 3, Pages 635-640Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11738-013-1441-4
Keywords
Acclimatization; Cassia angustifolia; In vitro conservation; Plantlet conversion; Synthetic seeds
Categories
Funding
- UGC under the scheme of Maulana Azad National Fellowship
- Council of Science and Technology (UP-CST) [CST/D 3836]
- UGC, Government of India, New Delhi [39-369/2010(SR)]
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The present study described the encapsulation of nodal segments of Cassia angustifolia Vahl. excised from 1-month-old in vitro raised cultures for short-term conservation and propagation. Various concentrations and combinations of gelling matrix (sodium alginate) and complexing agents (calcium chloride) were tested to prepare uniform beads. The ideal beads were obtained through a combination of 3 % sodium alginate and 100 mM calcium chloride. The maximum conversion response (94 %) of encapsulated beads was obtained in Murashige and Skoog's medium (MS medium) supplemented with 2.5 mu M benzyladenine (BA) and 0.4 mu M alpha-naphthalene acetic acid (NAA) after 6 weeks of culture. The encapsulated and non-encapsulated nodal segments were also stored at 4 A degrees C for different time periods (0, 1, 2, 4, 6 and 8 weeks). The regenerated microshoots were best rooted in optimized rooting medium that comprised half-strength MS + 1.0 mu M indole-3-butyric acid (IBA) + 5.0 mu M phloroglucinol (PG) for the production of complete plantlets. The regenerated plantlets were successfully hardened and acclimatized in natural conditions with 70 % survival rate.
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