Essential oils of Bidens tripartita L. collected during period of 3 years composition variation analysis

The aim of this study was to evaluate the variation of essential oils composition of Bidens tripartita L. collected during three successive years (2009-2011). Essential oils were obtained by supercritical CO2 extraction, applying gas chromatographic-mass spectrometric (GC-MS) analysis for identification of volatile compounds afterwards. The essential oils of B. tripartita showed a characteristic chemical composition from year to year, observing both quantitative and qualitative compounds differences. The yield of essential oils was 22 and 35 % higher in 2010 year material than in 2009 and 2011 year, respectively. The main compounds found in the B. tripartita essential oils were alpha-pinene (3.7-12.1 %), p-cymene (2.8-8.0 %), beta-ocimene (40.5-45.9 %), beta-elemene (9.9-15.6 %), iso and alpha-caryophyllenes (4.3-6.8 % and 5.2-8.2 %), and alpha-bergamotene (3.3-9.4 %). To determine the significance of changes in the identified compounds in the samples, representing different plant collection year, statistical hypothesis testing was applied. For classification of these samples to the groups and evaluation of similarity between them principal component analysis, multivariate analysis of variance and hierarchical cluster analysis techniques were used. The correlation analysis helped to find out the strength of linear relationship between amount of each compound and meteorological data (temperature and precipitations).