Journal
ACTA PHYSIOLOGIAE PLANTARUM
Volume 31, Issue 3, Pages 649-653Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11738-008-0250-7
Keywords
Encapsulation; Germplasm exchange; Synthetic seeds; Short-term storage; Toothache plant
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Funding
- Council of Scientific & Industrial Research (CSIR), New Delhi
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This article demonstrates the plantlet regeneration from alginate-encapsulated shoot tips of Spilanthes acmella. Shoot tip explants excised from in vitro proliferated shoots were encapsulated in calcium alginate beads. The best gel complexation for encapsulation of shoot tips was achieved using 3% sodium alginate and 100 mM calcium chloride. Maximum percent response for the conversion of encapsulated shoot tips into plantlets was obtained on growth regulator-free full-strength liquid MS (Murashige and Skoog, Physiol Plant 15:473-497, 1962) medium. The addition of MS nutrients in alginate matrix was found to have pronounced effect on shoot and root emergence from alginate beads. Encapsulated shoot tips could be stored at low temperature (4A degrees C) up to 60 days. Plantlets regenerated from encapsulated shoot tips were acclimatized successfully. The present synthetic seed technology could be useful in large-scale propagation as well as short-term conservation and germplasm distribution and exchange of Spilanthes acmella.
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