Journal
ACTA PHARMACOLOGICA SINICA
Volume 31, Issue 5, Pages 609-615Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/aps.2010.37
Keywords
cyclic bisbibenzyls; liverworts; apoptosis; prostate carcinoma; PC3 cells; Bcl-2; poly(ADP-ribose) polymerase
Funding
- National Natural Science Foundation of China [30772594]
- Shandong Provincial Key Research Foundation [2006GG1102023]
- Shandong Scientific Technology Program [2008GG10002042]
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Aim: To investigate the cytotoxic effects of four cyclic bisbibenzyls, Riccardin C (Ric), Pakyonol (Pak), Marchantin M (Mar), and Plagiochin E (Pla) against chemoresistant prostate cancer PC3 cells. Methods: Cell growth was assayed by MTT method, and apoptotic related protein Bcl-2 and Bax, poly(ADP-ribose) polymerase (PARP) were examined by Western blotting. Cell cycle and apoptosis of PC3 cells were evaluated with flow cytometry and morphologic examinations. Results: The four compounds inhibited proliferation and elicited cell death in a dose-and time-dependent manner with IC50 values of 3.22 mu mol/L for Ric, 7.98 mu mol/L for Pak, 5.45 mu mol/L for Mar, and 5.99 mu mol/L for Pla, respectively. Furthermore, exposed to these chemicals caused a decrease in the antiapoptotic protein Bcl-2 and an increase in proapoptotic Bax expression. PARP cleavage and caspase-3 activity were also observed. Conclusion: The results suggest that cyclic bisbibenzyls could be used for the development of novel therapeutic chemicals against prostate cancer.
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