Journal
NEURAL REGENERATION RESEARCH
Volume 5, Issue 15, Pages 1125-1129Publisher
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.3969/j.issn.1673-5374.2010.15.001
Keywords
magnetic resonance spectroscopy; mesenchymal stem cell; adipogenic differentiation; biomarker; lipid droplet
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Funding
- National Natural Science Foundation of China [30930027]
- Natural Science Foundation of Guangdong Province [8151503102000032]
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Nuclear magnetic resonance-visible mobile lipid, at 1.28 parts per million (ppm), is thought to be due to mobile lipid droplets formed in cells and has been considered unique for neural progenitor cells. However, this idea remains controversial. The present study examined the 1.28 ppm biomarker in other stem cells and non-stem cells, and explored the relationship between 1.28 ppm biomarker and mobile lipid droplets. H-1 nuclear magnetic resonance spectroscopy of EC109 cells, mesenchymal stem cells (MSCs) and adipogenic cells differentiated from MSCs was performed. Results show that 1.28 ppm biomarker was observed in human MSCs, but was absent from EC109 cells. Following adipogenic differentiation induced for 2 weeks, the 1.28 ppm biomarker climbed remarkably, with mobile lipid droplet generation, suggesting that the 1.28 ppm biomarker is not specific for neural progenitor cells because it is also observed in MSCs and adipogenic-induced differentiated cells. Moreover, it is possible to monitor MSCs differentiation following cell transplantation, using 1.28 ppm biomarker changes.
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