Journal
JOURNAL OF ROBOTICS AND MECHATRONICS
Volume 22, Issue 5, Pages 587-593Publisher
FUJI TECHNOLOGY PRESS LTD
DOI: 10.20965/jrm.2010.p0587
Keywords
lab-on-a-chip; spheroid; microrotation flow; hepatocyte
Categories
Funding
- Scientific Research of Priority Areas, System Cell Engineering, Multiscale Manipulation
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We propose three-dimensional (3D) spheroid formation involving perfusion and a lab-on-a-chip containing spheroid-forming chamber arrays. Cells are collected forming a spheroid in the chamber in micro-rotation. We previously reported a single chamber form hepatic spheroids 130 to 430 mu m in diameter, controlling size by varying chamber diameter and cell density. Here, we scaled the system up by a factor of 10 while maintaining size control of 180 +/- 30 mu m in diameter. Results were comparable to those using a single-chamber device. Long-term culture confirmed that cells in the spheroid maintained viability and diameters did not change after 24 hours. The system is readily applicable for creating size-controlled spheroids ensuring reliable, predictable in vitro data for drug screening and biological research.
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