Journal
ACTA HAEMATOLOGICA
Volume 129, Issue 4, Pages 197-206Publisher
KARGER
DOI: 10.1159/000345267
Keywords
Graft-versus-host disease; Mesenchymal stem cells; Stem cell transplantation; Placenta
Categories
Funding
- Basic Science Research Program of the National Research Foundation (NRF) of Korea
- Ministry of Education, Science and Technology [2011-0014079, 2012-007678]
- National Research Foundation of Korea [2011-0014079] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
Background and Aims: Immunomodulatory properties of mesenchymal stem cells (MSCs) have been applied to reduce the incidence of graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation (HSCT). Among the various sources of MSCs that have immunomodulatory effects in vitro, only placenta-derived MSCs (PD-MSCs) have not been evaluated in an in vivo model of GVHD. In this study, we investigated the immunomodulatory properties of PD-MSCs in vitro and evaluated their clinical potential for controlling GVHD in an animal model. Methods: A GVHD animal model was established by transplanting C57BL/6 donor bone marrow cells and spleen cells into lethally irradiated BALB/c recipient mice. To control GVHD, human PD-MSCs were transplanted into recipient mice (5 x 10(5) or 1 x 10(6) cells). Results: PD-MSCs suppressed mitogen-stimulated T cell proliferation in vitro in a dose-dependent manner. Moreover, PD-MSCs inhibited cytokine secretion (interleukin-12, tumor necrosis factor-alpha and interferon-gamma) of activated T cells. In vivo, the survival rate in the PD-MSC group (transplanted with 1 x 10(6) cells) was higher than that in the control group and histological scores were low in the PD-MSC group. Conclusion: We present the first evidence that human PD-MSCs can efficiently control GVHD in an HSCT in vivo model. Copyright (c) 2012 S. Karger AG, Basel
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available