Effects of some elicitors on tanshinone production in adventitious root cultures of Perovskia abrotanoides Karel

In this study, we investigated the effects of different biotic and abiotic elicitors including yeast extract (YE), methyl jasmonate (MJ), AgNO3 and sorbitol on biomass and production of cryptotanshinone and tanshinone IIA in adventitious roots cultures of Perovskia abrotanoides Karel. Elicitors had no significant effect on root dry weight. The highest cryptotanshinone and tanshinone IIA production was achieved with 200 mgfi YE and 25 mu M AgNO3, respectively. YE and AgNO3 were the most effective elicitors to stimulate the tanshinones production while the lowest concentration of MJ, only moderately promoted tanshinone accumulation. Sorbitol was almost ineffective in enhancing tanshinone content. Cryptotanshinone formation was stimulated more significantly by elicitation than tanshinone HA. The results suggested that elicitors have the ability to stimulate tanshinone content in adventitious roots culture of P. abrotanoides. (C) 2015 Elsevier B.V. All rights reserved.