Journal
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
Volume 66, Issue -, Pages 712-714Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1744309110014491
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [17380052, 20248010]
- Institute for Bioinformatics Research Development, Japan Science Technology Agency (BIRD-JST)
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Carbazole 1,9a-dioxygenase (CARDO) is the initial enzyme of the carbazole-degradation pathway. The CARDO of Novosphingobium sp. KA1 consists of a terminal oxygenase, a putidaredoxin-type ferredoxin and a ferredoxin-NADH oxidoreductase (Red) and is classified as a class IIA Rieske oxygenase. Red from KA1 was crystallized at 278 K by the hanging-drop vapour-diffusion method using PEG 4000. The crystal diffracted to 1.58 angstrom resolution and belonged to space group P3(2), with unit-cell parameters a = b = 92.2, c = 78.6 angstrom, alpha = gamma = 90, beta = 120 degrees. Preliminary analysis of the X-ray diffraction data revealed that the asymmetric unit contained two Red monomers. The crystal appeared to be a merohedral twin, with a twin fraction of 0.32 and twin law (-h, -k, l).
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